复合蛋白锌对病毒致损小鼠介绍.docVIP

复合蛋白锌对病毒致损小鼠 心肌损伤的保护作用 王振先 郝芳之 徐成伟 李劲松 张兆华 乔艳红 [摘要] 目的 探讨复合蛋白锌(CPZ)对病毒致损小鼠心肌损伤的保护作用及其生化机理。方法 以柯萨奇病毒B3亲心肌变异株（CVB3m）诱导的BALB/c心肌炎小鼠为研究对象，观察CPZ对小鼠的发病情况、血清SOD活性及活性氧浓度；并利用光镜及电镜观察了小鼠心脏的损伤情况。结果 服用CPZ组、CPZ合用VitC组、VitC对照组、感染对照组小鼠感染CVB3m后病死率分别为10%、10%、50%、90%；服用CPZ组、CPZ合用VitC治疗组较感染对照组病死率明显降低（p0.001），且稍低于VitC治疗组（p0.05）。CPZ组及CPZ合用VitC治疗组病理改变（坏死数、炎性浸润灶数）均较感染对照组及VitC组明显减轻（p0.001）。CPZ组及CPZ合用VitC治疗组血清SOD活性较感染组及VitC组明显增高，较正常组明显降低（p0.001），活性氧浓度较感染组及VitC组明显降低，较正常组明显升高。结论 氧自由基在心肌炎发病中起重要作用，病毒性心肌炎早期应用CPZ，可通过提高SOD活性，清除自由基（又称活性氧）来减轻心肌损害，降低感染动物的病死率，从而有效改善预后，且较VitC疗效好，为CPZ组治疗病毒性心肌炎的临床应用提供了可能性。 To investigate the protective effect and mechanism of compound protein zinc (CPZ) in mice cardiac muscle damage virus-induced in murine CVB3m myocarditis　Wang Zhenxian , Hao Fangzhi ,Xu Chengwei, Li Jinsong,Zhang Zhaohua ,Qiao Yanhong .　Department of Pediatrics, Second Hospital, Shandong University, Jinan 250033 【Abstract】　Objective:　To investigate the protective effect and mechanism of compound protein zinc (CPZ) in mice cardiac muscle damage caused by virus . Methods:　To observe SOD activities and oxygen free radical levels of acute myocarditis mice revulsive CVB3 ,and damnify of mice heart with microscope and electric microscope .Results: mortalities of CPZ ,CPZ plus VitC , VitC , in the infected control group are 10%,10%,50% and 90%, respectively; mortalities of CPZ , CPZ plus VitC groups depress clearly than in the infected control group，the mortalitie of CPZ group is well as CPZ and VitC group depress slightly than VitC group (p0.05). Pathological change（amount of myocardial cellular infiltration and myocardial necrosis）of all treated groups with CPZ clearly lighten than in the infected control and VitC group (p0.001). SOD activitis of all CPZ treated groups clearly elevated than in the infected control and VitC group, but clearly depress than normal group (p0.001)，the oxygen free radical levels clearly depress than in the infected control group and VitC group but elevated than normal gr