TRPC6在蛋白尿患者肾小球组织的表达和意义.pdfVIP

摘 要 目 的 分析TRPC6 在人类正常肾组织和蛋白尿患者肾组织的表达差异，以探讨 该分子在蛋白尿发生中的可能作用。方 法 收集83 例蛋白尿患者肾组织，用TRPC6 多克隆抗体对蛋白尿患者肾组织进行免疫组化染色，在计算机图像分析软件中测算 TRPC6 阳性面积比，对蛋白尿患者不同蛋白尿程度及不同病理类型下肾小球中TRPC6 的表达与临床、病理指标的关系进行分析。结 果 1.蛋白尿患者肾小球TRPC6 的阳 性面积比在不同的24 小时尿蛋白定量分级中存在统计学差异(P0.05)。2.蛋白尿患 者肾小球TRPC6 的阳性面积比在不同的病理类型中存在统计学差异(P0.05)，以在 Ⅲ级组为最高，Ⅳ级组最低。3. 蛋白尿患者肾小球TRPC6 在肾小球内的的表达水平 与24 小时尿蛋白定量呈正相关（r＝0.274，p=0.047）,与Scr 呈负相关（r＝-0.286， p=0.040）。结 论 1.蛋白尿患者肾小球TRPC6 的表达随着24 小时尿蛋白定量分级的 不同存在差异。2.蛋白尿患者肾小球TRPC6 的表达随着病理类型的不同存在差异。 3.TRPC6 的表达与临床指标的相关性提示：TRPC6 与患者尿蛋白及肾小球硬化有关。 4.TRPC6 的异常表达是导致肾小球疾病的因素之一，且与疾病炎症活动状态密切相 关。 关键词：瞬时受体电位阳离子通道蛋白6；蛋白尿；表达 Expression Profile and Significance of TRPC6 in Glomeruli from Patients with Proteinuria Abstract Objective Analysis the differential expression of TRPC6 in human normal nephridial tissue and in patients’ nephridial tissue with proteinuria to discuss the possible mechanism of TRPC6 in proteinuria. Methods 83 patients’nephridial tissue with proteinuria were collected and TRPC6 polyclonal antibody was used to do immunohistochemical staining to these samples.The positive area ratio of TRPC6 were calculated by computer image analysis software. Analysis the patients with proteinuria of different protein content and different pathological mechanism,and discuss the relation of TRPC6 expression with clinical and pathological index. Results 1.Statistical differences were existed in24h proteinuria in TRPC6 positive area ratio of patients with proteinuria(P0.05).2. Statistical differences were existed in diferent pathological mechanism of proteinuria patients with TRPC6 positive area ratio(P0.05),grade Ⅲ was the highest and grade Ⅳ was the lowest.3.Positive correlation were observed in