有机垃圾热解炭对紫色土细菌群落结构的影响.pdfVIP

中国环境科学 2017,37(2) ：669~676 China Environmental Science 有机垃圾热解炭对紫色土细菌群落结构的影响 * 张尚毅,刘国涛 ,谢梦佩 (重庆大学,三峡库区生态环境教育部重点实验室,重庆 400045) 摘要：以 700℃热解制得城市有机垃圾(OFMSW)生物炭,为研究该生物炭对氮素缺乏的紫色土有机质、氮营养元素以及微生物群落结构的 影响,设计了为期 12 个月的土壤盆栽培养试验.试验共设置生物炭添加量分别为 0%、1%、3%和 5%(w/w) 的 4 个处理.采用高通量测序技术 分析土壤的微生物群落结构.试验表明:3%和 5%的生物炭添加量显著提高了土壤有机质和全氮含量, 同时显著降低了紫色土中细菌的 α-多 样性;主成分分析显示,0%与 1%添加量处理的细菌群落组成相似,3%与 5%添加量处理的细菌群落组成相似. Illumina 测序从 24 个土样中鉴 定出了 42 门细菌,其中主要的 6 个菌门为变形菌门、酸杆菌门、拟杆菌门、放线菌门、绿弯菌门和芽单胞菌门,它们序列数占序列总数的 83.7%~94.3%.各菌门对不同的生物炭添加量响应不同.从 24 个土壤样本中鉴定出 642 个菌属,相对丰度大于 1%的有 105 个.部分主要菌属 对 3%和 5%的生物炭添加量的响应与 0%和 1%的不同. 关键词：城市有机垃圾(OFMSW) ；生物炭；紫色土；细菌群落结构 中图分类号：X705 文献标识码：A 文章编号：1000-6923(2017)02-0669-08 Influence of organic fraction of municipal solid waste-based biochar on microbial community structure in a purple * soil. ZHANG Shang-yi, LIU Guo-tao , XIE Meng-pei (Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China). China Environmental Science, 2017,37(2)：669~676 Abstract ：Organic fraction of municipal solid waste (OFMSW) was pyrolyzed at 700℃ to produce biochar, which was used to amend an organic and nitrogen deficient purple soil in Chongqing. Twelve month pot incubation experiments were conducted to investigate effects of the OFMSW-based biochar on the content of organic matter (OM) and total nitrogen (TN) as well as microbial community structure of the purple soil. The experiments were performed with four treatments of 0%, 1%, 3% and 5% (w/w) of biochar addition, and the microbial communities of the amended soils were analyzed by high throughput sequencing technology. The results showed that the addition of 3% and 5% of biochar significantly increased the content of OM and TN and red