盐胁迫对黄瓜根际土壤细菌群落结构和生长发育的影响-园艺学报.pdfVIP

园 艺 学 报 2010 ，37（5）：741–748 Acta Horticulturae Sinica 盐胁迫对黄瓜根际土壤细菌群落结构和生长发 育的影响 * 吴凤芝 ，包 静，刘淑芹 （东北农业大学园艺学院，哈尔滨 150030） 摘 要：以耐盐性不同的两个黄瓜品种‘津春 5 号’(耐盐)和‘津优 1 号’(盐敏感)为研究对象， 设置 4 个NaCl浓度处理(0、100、200 、300 mmol · L-1)，采用PCR-DGGE技术研究整个生长时期根际土壤 细菌群落结构的变化。结果表明：盐胁迫能显著降低根际土壤细菌群落的丰富度(P ＜0.05)，低盐处理下， 盐敏感品种根际细菌的丰富度较高；高盐胁迫下，耐盐品种根际细菌群落的丰富度较高。高盐胁迫对盐 敏感品种根际细菌群落结构组成影响较大。各时期DGGE 图谱差异条带的序列片段经测序比对，推测为4 大细菌类群：Firmicutes 、Proteobacteria 、Gemmatimonadetes和Bacteroidetes ，且大多属于Proteobacteria (变 形菌门)的α–变形菌纲和γ–变形菌纲。随着盐浓度的增加黄瓜的株高和单株产量依次降低。 关键词：黄瓜；盐胁迫；根际土壤；细菌群落结构；多样性 中图分类号：S 642.2 文献标识码：A 文章编号：0513-353X （2010 ）05-0741-08 Effects of Salt Stress on Rhizospheric Soil Bacterial Community Structure and Cucumber Yield * WU Feng-zhi ，BAO Jing ，and LIU Shu-qin （College of Horticulture，Northeast Agricultural University ，Harbin 150030 ，China ） Abstract ：In order to study effects of salt stress on changes of soil microbial quantities and the diversity of soil microbial community by the technology of PCR-DGGE, two kinds of cucumbers with different saline tolerance were selected，Jinchun 5 （salt-tolerant ）and Jinyou 1 （salt-sensitive ），and four concentrations of NaCl were designed （0，100，200 and 300 mmol · L-1 ）. The results showed that salt stress could reduce the richness of soil bacterial community significantly （P0.05 ）. The richness of salt-sensitive cultivar was high under low salt treatment, while the richness of salt-tolerant cultivar was high under high salt treatment. High salt stress had more effects on the rhizosphere bacterial community structure of salt-tolerant cultivar. Sequences of different bands of four stages belonged to four bacterial groups ，Firmicutes ，Proteobacteria ，Gemmatimonadetes and Bacteroidetes. Most of them belonged to Alphaproteobacteria and Gamm