基于12srrna和16srrna基因序列探讨中国蚤蝇科部分属间的系统.pdfVIP

动 物 学 研 究 2010，Aug. 31(4): 370−374 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI ：10.3724/SP.J.1141.2010.04370 基于 12S rRNA 和 16S rRNA 基因序列探讨中国蚤蝇科 部分属间的系统发育关系 赵丹彤 1, 2，刘广纯 2,* ，王剑峰 2 (1. 沈阳农业大学 植物保护学院，沈阳 110161 ；2. 沈阳大学 生物与环境工程学院，沈阳 110044) 摘要：基于线粒体12S rRNA 和16S rRNA 基因序列联合分析，采用最大简约法、最大似然法和贝叶斯法分 别构建了中国蚤蝇科 14 属的系统发育树。结果表明：联合分析序列总长度为819 bp，其中可变位点277 个，简 约信息位点200 个；A+T 平均含量为77.7%，具A 、T 偏倚性。系统发育分析显：中国蚤蝇科为单系发生，分为 蚤蝇亚科和裂蚤蝇亚科两个单系群。蚤蝇亚科内脉蚤蝇属、锥蚤蝇属和刺蚤蝇属亲缘关系较近，栅蚤蝇属与栓蚤 蝇属亲缘关系较近；裂蚤蝇亚科中虼蚤蝇属与裂蚤蝇属互为姐妹群，寡蚤蝇属与伐蚤蝇属互为姐妹群。 关键词：蚤蝇科；12S rRNA 基因；16S rRNA 基因；系统发育 中图分类号：Q969.451.8; Q349.1; Q951.3 文献标志码：A 文章编号：0254-5853-(2010)04-0370-05 Phylogenetic Relationships Among Some Chinese Genera of Phoridae Based on Mitochondrial 12S and 16S rRNA Gene Sequences ZHAO Dan-Tong1, 2, LIU Guang-Chun2, *, WANG Jian-Feng2 (1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China; 2. College of Biological and Environmental Engineering, Shenyang University, Shenyang 110044, China) Abstract: The phylogenetic relationships among 14 Chinese genera of Phoridae were reconstructed based on concatenated sequences of the mitochondrial 12S and 16S rRNA genes using maximum parsimony, maximum likelihood and Bayesian methods. The alignment of the concatenated sequences spanned 819 sites including 277 variable sites, of which 200 were parsimony-informative. The sequences showed a 77.7% A+T bias. The phylogenetic analysis showed that the Phoridae was monophyletic. It was shown that the involved genera in the study