出生后心肌细胞的增殖和其调控相关microRNAs的实验研究.pdf

出生后心肌细胞的增殖和其调控相关microRNAs的实验研究.pdf

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出生后心肌细胞的增殖和其调控相关microRNAs的实验研究

ABSTRACT………………………—1.£一—.I一—1.£.—.L一48828282858687l…‘‘…’42……………”5…9712………96 whenwhichwithinwerewerewerewerewithAndMethodsmicemicroRNAsmRNAsmiRNAsmiRNAsmostmiRNAsmiRNAsmiRNAsmiRNAsmitosesABSTRACTmammalianmRNAs.miRNAsmiRNAsmRNAsmechanismsmiRNAsbecomePartPatternPostnatal一notratsratshaveDrolfferationasareRecentretainedneonatalRNAsRNAsnumerousrelatedrolesknownroles.ofrolesrevealedroleRatRats一‘一ondbirth.TheCanceaseTheandaferactionsaboutactionandandandandandandandandratrole0nofoftenofofofofofolderResearcheSareexacttoexerttofunctionalforandandabundanceand’一’‘cardiaccelldifferentiatedcertaincelldifferentiationdetaildissecteddemonstratecelldoublethattheevidentthattheirthistotheirthethethedemonstratedcharacterizedthethethatthethethethethethatthecard1~studiesseveraldurin23untranslatedforstudieslife.Thelocatedterminalinisitisindividualinisininininisininvolvedininininidentifiedin‘ withwerewaswerewaswithinweekswaswithwerewerewerewaswaswerewerewhichwerewithWasMainmitoticmitoticmiRNAsmicroRNAmiRNAsmRNAsmainmiRNAmiRNAsmiRNAsmiRNAsmRNAsmiRNAmetabolicmiRNAsmRNAsH3PH3PH3PKi-67Ki-67Ki一67birth.P2betweenP2P2P2P2P2P2betweenbetweenversusratsratsheartshasToGlobalGOGeneP4WP4WP4WP4WP4W.P4WP4WP4WP4WnumbernumberRNAknownCorrelationbetweenbetweenbetweenoron44.Bioinformatic4.1.Functional4.2.reduction.Mainatatrats.Wecurve5andalmostandafterSDand95alsoassessedalsoandandatandacidacidandofofof0ofofofof65ofofofofofNCBI.ofofoxidationofassociatedassociatedassociated2842totototoforfoundforfromforfromfromfunctionfunctionalfunctionfunctionandandandandandandandcalculateddrawdecreased3-folddecreasedclustered353correlationdifferentialcellthetotaltotalextractedthattimethethethedetermineddecreasedclassificationthethethethethisthatthethethethethethethantransition.Ashowedsameseverallowerleast7.neimmunofluorescenceinindicesinininvolvedininincreasedinininidentifiedinisinvolved11 werewerewereweekswaswaswaswaswithwithweeksweeks.AMicroRNA-GO-NetworkMethodsMTSmicroRNAsmiRNAmRNAmiRNAsmiRNAsmRNAs.ThemostmicroRNAmiRNAmRNAm

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