about cryoprecipitate preparation and quality influencing factors（关于冷沉淀剂制备和质量影响因素）.docVIP

About cryoprecipitate preparation and quality influencing factors [Keywords] cryoprecipitate; preparation; quality Cryoprecipitate, fresh frozen plasma was prepared from a composition of blood, is mainly used for hemophilia A, von Willebrand disease, fibrinogen deficiency patients can also be used for post-operative bleeding, severe trauma and DIC [ 1]. cryoprecipitate factor Ⅷ unstable factor affected by many factors, so each bag of cryoprecipitate factor Ⅷ content varies, now the center of cryoprecipitate preparation and quality factors affect the analysis reported below. 1 Materials and methods 1.1 Materials and instruments Large-capacity centrifuge at low temperature (CrYOFUge6000), quick-frozen refrigerator (SanYO), produced by Japan’s Hitachi, the U.S. CYTOTHERM produced CT-4T.6C type automatic swing constant temperature water bath melt tank, Japanese clotting factor analysis SYSMaX (CA-50) instrument Korean heat sealing machine (SE250). 1.2 cryoprecipitate raw slurry preparation Fresenius Kabi (Guangzhou) production confined quadruple bag, collecting 400 ml of whole blood centrifuged within 6h made plasma 60 bags (200 ± 10ml), the plasma is rapidly set to -80 ° C Freezer freeze rear -30 ℃ refrigerator alternate. 1.3 Preparation of cryoprecipitate Take placed in 60 bags of plasma at room temperature 3min, to be connected to two bags of plastic pipe softened and divided into two groups (A, B) into the low-temperature water bath (4 ?C to 6 ?C) A group of 30 bags with a water bath siphon Preparation of cryoprecipitate, B group 30 bags prepared by centrifugal method with rapid melting of cryoprecipitate, timing. A group of fresh frozen plasma melting fixed thermostat swinging water bath of blood bags in the folder, vertically immersed in a constant temperature circulating water bath, water bath automatically swing, plasma melting supernatant siphon into the empty bag, bags center remaining (25 + -5) ml did not melt in the plasma and a