about three fluorescence marker of the short double-stranded rna in vitro transfection characteristics comparative（大约三个短的荧光标记双链rna体外转染特点比较）.docVIP

About three fluorescence marker of the short double-stranded RNA in vitro transfection characteristics Comparative Author: Ma Yun, COMMITTED, Ruled, Wen An Min, Gao Jun, ZHOU Can-quan, Chen Gu [Abstract] Objective: To compare three different fluorescently labeled short double-stranded RNA (siRNA) transfection in vitro characteristics: using liposomes LipofectamieTM2000 transfected three fluorescence marker siRNA into mouse embryonic fibroblast cell line NIH 3T3 flow cytometer observed transfection efficiency the ordinary inverted fluorescence microscope quenching, laser scanning confocal microscope to observe the distribution within the cell. results: three fluorescent under the same conditions of transfection siRNA speed into the cell, intracellular different positioning, different transfection efficiency, three fluorescently labeled antifade have different capacities. Conclusion: fluorescence-labeled siRNA can play a good tracer reference as the distribution of the fluorescence of siRNA and transfection efficiency with the fluorescent-labeled siRNA to take into account the selection of fluorescently labeled siRNA with no fluorescently labeled siRNA differences. [Keywords] fluorescently labeled siRNA transfection [Abstract] AIM: To compare the transfection character of three different fluorescently labeled small interfering RNA (siRNA) in vitro. METHODS: Three fluorescently labeled siRNA were transfected with LipofectamieTM2000 into NIH 3T3 cells for moni  toring their transfection efficiency with flow cytometry, tracking their delivery with confocal microscopy and checking their photostability with fluorescent microscope. RESULTS: These three fluorescently labeled siRNA varied in intracellular distribution, transfection efficiency and quench speed. CONCLUSION: The fluorescence labeled siRNA helps to tracking the delivery of siRNA in cells. The difference between fluorescently labeled siRNA and unlabeled siRNA should be considered when the fluoresce