pet21b载体（Pet21b vector）.docVIP

pet21b载体（Pet21b vector） The pET21b vector sequence of Genbank format is as follows: Please send the following sequence copy pasted into Notepad, and then save the file as pet21b, you can use the NTI software in the sequence into Vector. At the same time we sold pET21b plasmid, the price is 400 yuan, contact I37-649-32O-37 LOCUS pET-21 B (+) 5442 BP DNA Circular SYN 26-Nov-2011 DEFINITION pET-21 B (+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers Source 1..5442 /organism= pET-21 B (+) /mol_type= other DNA Terminator complement (1..129) /label= T7_terminator Terminator complement (1..129) /label= T7_terminator Misc_feature 69..87 /label= T7_Terminal_primer Misc_feature complement (140..157) /label= 6xHis Misc_feature complement (206..238) /label= T7_gene10_leader Misc_feature complement (206..238) /label= T7_leader Misc_feature complement (221..239) /label= Xpress_fwd_primer Misc_feature complement (247..263) /label= T7_transl_en_RBS Misc_feature complement (281..308) /label= lacO Promoter complement (308..326) /label= T7_promoter Misc_feature complement (410..429) /label= pBRrevBam_primer Gene complement (358..621) /label= tet (564-300) /gene= tet (564-300) Misc_feature 704..1795 /label= lacI CDS 836..1795 /label= ORF frame 2 /translation= MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ* Gene complement (1854..2129) /label= tet (852-576) /gene= tet (852-576) Misc_feature 2604..2795 /label= ROP misc_feature补充（2789。2811） 标签=“pgex_3_primer” rep_origin补充（3210。3829） 标签=“pbr322_origin” CD（3984。4844）补充 标签=“Ampicillin” /基因=“Ampicillin” /注=“ORF框3” 翻译=“msiqhfrvalipffaafclpvfahpetlvkvkdaedqlgarvgy ieldlnsgkilesfrpeerfpmmstfkvllcgavlsridagqeq