D_101_型树脂对芍药苷吸附分离性能的研究.pdf

122 Vol. 12,No. 2 20062 Chinese Journal of Experimental Traditional Medical Formulae Feb. ,2006 101 * 桂双英 , 周亚球, 柯仲成 ( 安徽中医学院药学院药剂教研室, 安徽 合肥 230031) [ ] :: HPLC ; : 101: 25mg mL36BVh, 322mgg,20%9125%, 7370% : 101 [] ;; ;; [] R284. 1 [ ] B [ ] 2006 Adsorption and Separation Properties of D101 Resin for Purification of Paeoniflorin GUI Shuangying, ZHOU Yaqiu , KE Zhongcheng ( School of Pharmacy , Anhui College fo TCM,Hfe ei 230031, China) [Abstract] Objective:To study the separation effect of 101 macroporous resin for the purification of Paeoniflorin in Ra ix Paeonia .Methods:HPLC was used to analyze the content of Paeoniflorin, and the adsorption performance and the elution parameters were investigated.Results:The optimal separation conditions were as follows: the concentration of Paeoniflorinwas 2. 5mgmL with a flow rate of 3. 6BVh, and 20% alcohol was used as eluant. The adsorption of Paeoniflorin was 32.2mgg, and the elution ratio of Paeoniflorin was 91.25%, and thepurity of Paeoniflorin reached 73. 70% .Conclusion: 101 resin can be used to refine the Paeoniflorin in the extraction of Ra ix Paeonia. [Key words] Macroporous resin; Ra ix Paeoniae Rubra; Paeoniflorin; Adsorption; Separation ,, ,, , , , 1 [ 1] , LC10A ( ) ; , Paeonia lactfi lora Pall. (, [2] [ 1, 3] , ) , , ; 101( [4] ) ; , , 101 2 VPO S 46mm 150mm () ; - [ ]