应用正交实验法研究濒危中药白及成苗因子.pdf

29 4 Vol.29 No.4 2 0 1 2 8 Aug.2012 Experimental studies on factors affecting plantlets proliferation in vitro of Bletilla striata by orthogonal design 1 2 3 4 1 1 FANG Huiyong , LI Minhui , YAO Jianxun , SHI Lin , JIA Caifeng , ZHAO Qing ( 1. College of Traditional Chinese Medicine, Hebei University, Baoding 071002, China; 2. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014060, China; 3.Guizhou Yongchang TCM development Co., Ltd., Guiyang 550018, China; 4. Laoting County Hospital, Laoting 063600, China) Abstract: Objective To explore the factors affecting plantlets proliferation in vitro of Bletilla striata, and the effects of activated charcoal on plantlets growth. Methods Orthogonal array experimental design was applied to study the effects of TDZ, NAA, inositol on plantlets proliferation. Results Plantlets proliferation was signifi cantly affected by TDZ and inositol. Optimal media to induce plantlets proliferation was Fast medium with TDZ 5.0 mg/L and NAA 0.05 mg/L and inositol 50 mg/L. an