mRNA差别显示技术在植物发育研究中的应用_闫桂平.pdfVIP

　2001, 18 (1):52 ～57 Chinese Bulletin of Botany mRNA ① 1,2 1 2 1 闫桂平　 马凤鸣　 韩天富　 李文华 1 (, 150030) 2 (, 100081) 　m RNA 。 、, 。 、、、、、、。 　m RNA , , Applications of mRNA Differential Display in the Studies on Plant Development 1,2 1 2 1 YAN Gui_Ping 　MA Feng _Ming 　HAN Tian_Fu 　LI Wen_Hua 1 (Department of Agronomy , NortheastAgricultural University, Harbin 150030) 2 (Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081) Abstract　mRNA di erential display technique is an e ective method to analyze di erential gene expression and to seach the genes which expressed specially .This paper brie ly review s the recent progress o applications o this method in research on plant developmental, such as the molecular mechanism o vernalization, photoperiod response, the development o root, lower and seed, male sterility, senescence, ruit maturation, etc . Key words　mRNA di erential display, plant development, application mRNA (mRNA di erential display PCR)Liang (1992 ) 。, mRNA 3′poly (A ),,mRNA:cDNA 。, cDNA ,PCR , 。 、 、、、, , (Bauer , 1993;Oh , 1995;Chen Peek, 1996;Zegzouti , 1997)。7 , 、、、、、 。,、 、、。 。 ① 863 。 :, , 1968 , 1994 , , 。3 , 12 。 :1999-10-28　　:1999-12-29 　　:　 1 :mRNA 　　　 53 1　 。(1998) mRNA ,1 20d cDNAVRC54。Northern VRC54 1 ,, 。 ,。 ,Sage_Ono (1998) mRNA ,cDNA pnc401, pnc401 mRNA ,。(1998), 58S 58 , mRNA , 58S 3 cDNA, cDNA 。 2　 。Ogihara (1998) cDNA,29 , Northern 14 cDNA ,。Hamano (1998) 。0,2,4,7, 10, 13d ,11 , 2 cDNA sgr101 sgr701 、 。Watillon (1998), cDNA ,md-f s1 md-f s , 。 3　 mRNA 。、F1 mRNA mRNA 、