a highly conserved gene island of three genes on chromosome 3b of hexaploid wheat diverse gene function and genomic structure maintained in a tightly linked block一个高度保守的基因岛三个基因3 b染色体上六倍体小麦不同基因功能和基因结构中维护一个块紧密相连.pdfVIP

Breen et al. BMC Plant Biology 2010, 10:98 /1471-2229/10/98 R E S E A R C H A R T I C L E Open Access Research article A highly conserved gene island of three genes on chromosome 3B of hexaploid wheat: diverse gene function and genomic structure maintained in a tightly linked block 1,2 3 4 4 1,5,6 7 7 James Breen , Thomas Wicker , Xiuying Kong , Juncheng Zhang , Wujun Ma , Etienne Paux , Catherine Feuillet , Rudi Appels*1 1 and Matthew Bellgard Abstract Background: The complexity of the wheat genome has resulted from waves of retrotransposable element insertions. Gene deletions and disruptions generated by the fast replacement of repetitive elements in wheat have resulted in disruption of colinearity at a micro (sub-megabase) level among the cereals. In view of genomic changes that are possible within a given time span, conservation of genes between species tends to imply an important functional or regional constraint that does not permit a change in genomic structure. The ctg1034 contig completed in this paper was initially studied because it was assigned to the Sr2 resistance locus region, but detailed mapping studies subsequently assigned it to the long arm of 3B and revealed its unusual features. Results: BAC shotgun sequencing of the hexaploid wheat (Triticum aestivum cv. Chinese Spring) genome has been used to assemble a group of 15 wheat BACs from the chromosome 3B physical map FPC contig ctg1034 into a 783,553 bp genomic sequence. This ctg1034 sequence was annotated for biological features such as genes and transposable elements. A three-gene island was identified among 80% repetitive DNA sequence. Using bioinformatics analysis