a method for non-invasive genotyping of apcmin+ mice using fecal samples一种非侵入性的方法pcr apcmin +老鼠使用粪便样本.pdfVIP

Symonds and Fenech Biological Procedures Online 2012, 14:1 /content/14/1/1 Biological Procedures Online METHODOLOGY Open Access A method for non-invasive genotyping of APCmin/+ mice using fecal samples Erin L Symonds1,2* and Michael Fenech1 Abstract The APCmin/+ mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non- invasive method of genotyping may be applied to other transgenic mouse models. Keywords: APCmin/+, feces, genotyping, cancer, non-invasive Introduction mice and their wild-type littermates with DNA isolated With advances in molecular genetics, there are large from their fecal samples. numbers of transgenic and knock-out mice being devel- oped. The most common method for genotyping these Results and discussion mice is with DNA isolated from tail, ear or toe tissue, or This study has shown that APCmin/+ mice and their from blood. These collection techniques however are wild-type litter mates ( 3 wk) can be genotyped using invasive procedures and are not appropriate for repeat DNA isolated from whole fecal samples without the sampli