a new flavone c-glycoside from clematis rehderiana一个新的黄酮从铁线莲rehderiana c-glycoside.pdfVIP

Molecules 2010, 15, 672-679; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication A New Flavone C-Glycoside from Clematis rehderiana Zhi-Zhi Du 1, Xian-Wen Yang 2, Hao Han 1, Xiang-Hai Cai 1 and Xiao-Dong Luo 1,* 1 State Key Laboratory of Phytochemistry and Plant Resources of West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; E-Mail: duzhizhi@ (Z.-Z.D.) 2 Key Laboratory of Marine Bioresources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; E-Mail: yangxw76@163.com (X.-W.Y.) * Author to whom correspondence should be addressed; E-Mail: xdluo@. Received: 24 December 2009; in revised form: 28 January 2010 / Accepted: 29 January 2010 / Published: 29 January 2010 Abstract: A new flavone C-glycoside, isovitexin 6″-O-E-p -coumarate (1) and two known flavonoid glycosides—quercetin 3-O-β-D-glucuronopyranoside (2) and isoorientin (3)— were isolated from an ethanol extract of aerial parts of Clematis rehderiana. Their structures were determined by spectroscopic methods. The antioxidant effects of the two flavone C-glycosides were evaluated by both the MTT and DPPH assays. Compound 1 showed potent activities against H O -induced impairment in PC12 cells within the 2 2 concentration range tested, whereas compound 3 scavenged DPPH radical strongly, with an IC50