a tourist-like mite insertion in the upstream region of the bnflc.a10 gene is associated with vernalization requirement in rapeseed (brassica napus l.)一个tourist-like螨插入bnflc上游地区。.pdfVIP

Hou et al. BMC Plant Biology 2012, 12:238 /1471-2229/12/238 RESEARCH ARTICLE Open Access A Tourist-like MITE insertion in the upstream region of the BnFLC.A10 gene is associated with vernalization requirement in rapeseed (Brassica napus L.) 1 1 2 1 1 1 1 1 Jinna Hou , Yan Long , Harsh Raman , Xiaoxiao Zou , Jing Wang , Shutao Dai , Qinqin Xiao , Cong Li , 3 4,5 1* Longjiang Fan , Bin Liu and Jinling Meng Abstract Background: Rapeseed (Brassica napus L.) has spring and winter genotypes adapted to different growing seasons. Winter genotypes do not flower before the onset of winter, thus leading to a longer vegetative growth period that promotes the accumulation and allocation of more resources to seed production. The development of winter genotypes enabled the rapeseed to spread rapidly from southern to northern Europe and other temperate regions of the world. The molecular basis underlying the evolutionary transition from spring- to winter- type rapeseed is not known, however, and needs to be elucidated. Results: We fine-mapped the spring environment specific quantitative trait locus (QTL) for flowering time, qFT10-4, in a doubled haploid (DH) mapping population of rapeseed derived from a cross between Tapidor (winter-type) and Ningyou7 (semi-winter) and delimited the qFT10-4 to an 80-kb region on chromosome A10 of B. napus. The BnFLC.A10 gene, an ortholog of FLOWERING LOCUS C (FLC) in Arabidopsis, was cloned from the QTL. We identified 12 polymorphic sites between BnFLC.A10 parental alleles of the TN-DH population in the upstream region and in intron 1. Expression of both BnFLC.A10 alleles decreased during vernalization, but decreased more slowly in th