adult mesenchymal stem cells and cell-based tissue engineering成人间充质干细胞和细胞组织工程.pdfVIP

Arthritis Research and Therapy Vol 5 No 1 Tuan et al. Review Adult mesenchymal stem cells and cell-based tissue engineering Rocky S Tuan, Genevieve Boland and Richard Tuli Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA Corresponding author: Rocky S Tuan (e-mail: Tuanr@) Received: 7 October 2002 Accepted: 1 November 2002 Published: 11 December 2002 Arthritis Res Ther 2003, 5:32-45 (DOI 10.1186/ar614) © 2003 BioMed Central Ltd (Print ISSN 1478-6354; Online ISSN 1478-6362) Abstract The identification of multipotential mesenchymal stem cells (MSCs) derived from adult human tissues, including bone marrow stroma and a number of connective tissues, has provided exciting prospects for cell-based tissue engineering and regeneration. This review focuses on the biology of MSCs, including their differentiation potentials in vitro and in vivo, and the application of MSCs in tissue engineering. Our current understanding of MSCs lags behind that of other stem cell types, such as hematopoietic stem cells. Future research should aim to define the cellular and molecular fingerprints of MSCs and elucidate their endogenous role(s) in normal and abnormal tissue functions. Keywords: cell differentiation, cell signaling, mesenchymal stem cells, stem cells, tissue engineering Introduction cations depends critically upon a thorough understanding Despite the pluripotency of embryonic stem (ES) cells, of the biology of t