同位素示踪法（Isotope tracer method）.doc

同位素示踪法（Isotope tracer method） Application of isotope tracer method in biology experiment in Senior High School The isotope tracer method is the use of radionuclide tracer labeled as trace analysis method to the research object, namely the radioactive isotope atoms to other substances to participate, let them move together, then the migration of radioactive detecting instrument for tracking, you can know what the radioactive atom movement path, where, what is the distribution of. The isotope tracing method is an important method is often used in biological experiments, it can be the source, the study of the intracellular elements or compounds of the composition, distribution and fate, and to understand the cellular structure and function, chemical change and reaction mechanism. Radioisotope used in tracer technology is generally used as an important element in the formation of cell compounds such as 3H, 14C, 15N, 18O, 32P, 35S, 131I, etc.. In high school biology textbooks, there are many related to the application of radioisotopes. The following are summarized as follows: 1 study the material and process of protein or nucleic acid synthesis The reflective atomic parameters to synthesis of protein or nucleic acid material (amino acid or nucleotide), let them move together, then the migration of radioactive detecting instrument for tracking, you can know what the radioactive atom path, where and how the distribution of sports. 2 to study the synthesis and transport of secreted proteins Using leucine labeled with 3H to investigate the synthesis, transport and secretion pathway of secretory proteins in cells. Given the premise of radiolabelled amino acids in disposable, by radioactive substances in cells were detected in different time position, you can clearly see that the organelle in the secretory protein synthesis and transport effect. For example, after the synthesis of protein in the endoplasmic reticulum attached to the ribosome through experiments that the secret