hepatoma cell-specific binding phage random peptide library screening and identification of peptide（肝癌特异性结合的噬菌体随机肽库筛选和肽的识别）.docVIP

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hepatoma cell-specific binding phage random peptide library screening and identification of peptide（肝癌特异性结合的噬菌体随机肽库筛选和肽的识别）.doc

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hepatoma cell-specific binding phage random peptide library screening and identification of peptide（肝癌特异性结合的噬菌体随机肽库筛选和肽的识别）

Hepatoma cell-specific binding phage random peptide library screening and identification of peptide Thesis network: Author: Zhang Xu Peng Jian Wang Shunwei Yang Pu Korea Angles Zhang Yang Germany [Abstract] Objective: phage random peptide library to screen a human hepatoma cell binding peptide, and its specific binding capacity of liver cancer cells, the biological identification methods: L 02 negative abatement cells, HepG2 target cells random phage heptapeptide library 4 Abatement screening. and 26 positive phage clones were randomly picked for sequencing and homology analysis. identified by ELISA binding activity of the phages with HepG2 cells by immunocytochemical staining, the immune staining to identify the binding specificity of H3 phage clones and hepatoma cells. synthetic peptide HBP3 immunofluorescence technique to observe the binding specificity of liver cancer cells. Results: 4 screened the phage in the target cell line HepG2 is significantly enriched in the 26 positive plaques were picked and phage monoclonal peptide insert sequence by sequencing, and ultimately produce five amino acid sequence by BLAST analysis revealed that, with these short peptide sequences in the protein database did not find good homology to the protein molecules by ELISA were identified, five positive clones in HepG2 and L-02 cells on the differences in binding, H3 phage clones with the difference of the two cells. immunocytochemical staining and immunohistochemistry H3 phage clones with targeting liver cancer cells was significantly higher than control cells confirmed the immunofluorescence color FITC-HBP3 can specifically bind to liver cancer cell membrane and the perinuclear cytoplasm Conclusion: heptapeptide HBP3 H3 phage clones presented with target cells is high affinity binding to lay an experimental foundation for the further development of high-targeting drugs for the treatment of liver cancer. [Key Words] liver tumors, tumor cells, phage display peptide lib