DNA芯片技术在动物医学中的应用研究进展.pdfVIP

中国农学通报 2010,26(14):27-30 Chinese Agricultural Science Bulletin DNA 芯片技术在动物医学中的应用研究进展 马艳平，陈豪泰，马丽娜，周建华，张 杰，丁耀忠，王 猛，刘文倩，刘永生 （中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室 农业部畜禽病毒学重点开放实验室，兰州730046） 摘 要：随着基因组数据的增长，DNA 芯片(DNA Chip)技术得到广泛应用。其集成化、微型化、自动化的 优点，为生物、医学、化学等领域的研究提供了一个强有力的工具，笔者从基因表达谱研究、病原微生物 检测、细菌分型、基因突变和多态性检测、病原微生物基因组学研究多个方面概述了DNA 芯片技术在动 物医学中的应用进展、并对DNA 芯片技术的原理和分类进行综述。 关键词：DNA 芯片；动物医学；原理；应用；分类 中图分类号：S858.28 文献标志码：B 论文编号：2010-0474 Research Progress on Application of DNA Chip in Animal Medicine Ma Yanping, Chen Haotai, Ma Lina, Zhou Jianhuan, Zhang Jie, Ding Yaozhong, Wang Meng, Liu Wenqian, Liu Yongsheng (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046) Abstract: With the increase of genome’s numbers, DNA Chip technology gained widely application. Its merit of integration, miniaturization, and automation advantages supply a powerful tool in the field of biology, medicine, chemistry and other research. This article overview DNA chip technology from gene expression spectrum research, identification of pathogenic microbial detection, bacterial typing, genetic mutations and polymorphism detection, pathogenic microbial genomics research in animal medicine application progress, and the principle of DNA chip technology are reviewed. Key words: DNA Chip; animal medicine; principle; application; classification 0 引言 光