complete chloroplast genome of oncidium gower ramsey and evaluation of molecular markers for identification and breeding in oncidiinae完成金蝶兰属植物的叶绿体基因组高尔半岛拉姆齐和评估在oncidiinae鉴定和育种的分子标记.pdfVIP

Wu et al. BMC Plant Biology 2010, 10:68 /1471-2229/10/68 R E S E A R C H A R T I C L E Open Access Research article Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae †1 †1 1 1 1 2 3 Fu-Hui Wu , Ming-Tsair Chan , De-Chih Liao , Chen-Tran Hsu , Yi-Wei Lee , Henry Daniell , Melvin R Duvall and Choun-Sea Lin*1 Abstract Background: Oncidium spp. produce commercially important orchid cut flowers. However, they are amenable to intergeneric and inter-specific crossing making phylogenetic identification ve ry difficult. Molecular markers derived from the chloroplast genome can provide useful tools for phylogenetic resolution. Results: The complete chloroplast genome of the economically important Oncidium variety Onc. Gower Ramsey (Accession no. GQ324949) was determined using a polymerase chain reaction (PCR) and Sanger based ABI sequencing. The length of the Oncidium chloroplast genome is 146,484 bp. Genome structure, gene order and orientation are similar to Phalaenopsis, but differ from typical Poaceae, other monocots for which there are several published chloroplast (cp) genome. The Onc. Gower Ramsey chloroplast-encoded NADH dehydrogenase (ndh) genes, except ndhE, lack apparent functions. Deletion and other types of mutations were also found in the ndh genes of 15 other economically important Oncidiinae varieties, except ndhE in some species. The positions of some species in the evolution and taxonomy of Oncidiinae are difficult to identify. To identify the relationships between the 15 Oncidiinae hybrids, eight regions of the Onc. Gower Ramsey chloroplast genome were amplified by PCR for phylogenetic analysis. A t