pcr假阴性和假阳性（PCR false negatives and false positives）.docVIP

pcr假阴性和假阳性（PCR false negatives and false positives） False negatives showed no amplification bands The key steps of PCR reaction include preparation of template nucleic acids, quality and specificity of primers, quality of enzymes and PCR cycle conditions. The reasons should also be analyzed and studied in view of the above aspects. The template template: contains protein, containing Taq enzyme inhibitor of the template, the template protein digestion without addition to the net, especially in the chromosome set of proteins in the extraction when excessive loss, or inhalation of phenol. Templat