分光光度法测定刺五加茎中黄酮含量（Determination of flavonoids in Acanthopanax senticosus stem by Spectrophotometry）.docVIP

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分光光度法测定刺五加茎中黄酮含量（Determination of flavonoids in Acanthopanax senticosus stem by Spectrophotometry）.doc

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分光光度法测定刺五加茎中黄酮含量（Determination of flavonoids in Acanthopanax senticosus stem by Spectrophotometry）

分光光度法测定刺五加茎中黄酮含量（Determination of flavonoids in Acanthopanax senticosus stem by Spectrophotometry） Spectrophotometric Determination of flavonoids in Acanthopanax senticosus stem.Txt, if I am poor enough to leave a bowl of rice, I will let you eat first, all the best things in the world should be mine, including you!! Can I say first love die? Come on, listen. Have the ability to take care of yourself, or honestly let me take care of you! Determination of flavonoids in Acanthopanax senticosus stem by Spectrophotometry Update Date: 2010-12-22 Click: Wang Liying, Song Yuping, Wang Shuzhen Taking rutin as the standard, the flavonoids in Acanthopanax senticosus stem were determined by spectrophotometry. Continuous reflux extraction was carried out with methanol as solvent. The regression equation was A=9.4000 C0.0320 gamma =0.9998 (n=5), the average recovery was 98.77%, RSD was 1.34%. Keywords spectrophotometry; Acanthopanax senticosus; stem; content; The stem of Acanthopanax senticosus is the stem of Acanthopanax senticosus Acanthopanax, senticosus (Rupr.et, Maxim) Harms. The content of flavonoids in the stems of Acanthopanax senticosus was determined by spectrophotometry. The result was satisfactory. 1 instruments and reagents UV-7530 ultraviolet spectrophotometer (Shanghai analytical instrument factory); other reagents are analytical pure; Acanthopanax senticosus stem is small Xingan ridge wild resources. The reference substance of rutin is provided by the China Institute of drug control (lot number: 080-9303) 2 methods and results 2.1 selection of experimental conditions 2.1.1 sample preparation: precision weighing medicinal powder 2 g, a Soxhlet extractor, petroleum ether defatted with 100 ml of 2 h, 90 mL and 4 h methanol extraction, 100 ml flask, methanol capacity, precision from 25 ml 50 ml volumetric flask with distilled water dilute to volume. It is. 2.1.2 standard preparation: weigh accurately in rutin 120 DEG decompression drying to constant weight control of