熟地黄炮制九蒸九晒过程中药效化学成分量变化及炮制辅料-MDPI.PDFVIP

752 Chinese Traditional and Herbal Drugs 47 5 2016 3 * 030619 HPLC Hypersil GOLD aQ C18 250 mm4.6 mm5 μm 1 mL/min25 -0.1%199210 nmA -0.1%397 203 nmD -0.1%496203 nm -0.1%1684334 nm5-5-HMF-0.1% 1189284 nmAccurasil NH2 250 mm4.6 mm5 μm -7228 1 mL/min25 ELSDAlltech2000ES93 2.6 L/minSPSS 20.0 -- 12 t- A D 5-HMF 3 4 6 A D 5- R283.1 A 0253 - 2670(2016)05 - 0752 - 08 DOI: 10.7501/j.issn.0253-2670.2016.05.011 Content changes of chemical components and their effect of adjuvants during the process of Rehmanniae Radix Praeparata (steamed for nine times and shined for nine times) MENG Xiang-long, MA Jun-nan, ZHANG Shuo-sheng, LI Kun, XUE Fei-fei, ZHANG Jie, YUAN Zhi-cui, TIAN Ye-fei, WANG Ming-fang Institute of Pharmaceutical Food Engineering, Shanxi University of Traditional Chinese Medicine, Jinzhong 030619, China Abstract: Objective By HPLC, to explore the differences of adjuvants for the changes of chemical components of Rehmanniae Radix Praeparata during process by steamed for nine times and shined for nine times. Methods HPLC was conducted on a Hypersil GOLD aQ C column (250 mm × 4.6 mm, 5 μm), flow rate was 1 mL/min, and the column temperature was 25 . With the mobile phase of 18 acetonitrile and 0.1% phosphoric acid water (199), the detection wavelength was 210 nm (catalpol), 203 nm (rehmannioside A, rehmannioside D, and leonuride), acetonitrile and 0.1% phosphoric acid water (397 and 496); 334 nm (actecosode), acetonitrile and 0.1% phosphoric acid water (1684), and 284 nm (5-HMF), acetonitrile and 0.1% phosphoric acid water (1189). Sugars was conducted on an Accurasil NH2 column (250 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrile and 0.1% phosphoric acid water (7228), fl