a complete set of nascent transcription rates for yeast genes一套完整的新生的酵母基因的转录率.pdfVIP

A Complete Set of Nascent Transcription Rates for Yeast Genes 1¤ ´ ´ 2 ´ ´ ´ 1 Vicent Pelechano , Sebastian Chavez , Jose E. Perez-Ortın * ´ ´ ´ ` ´ 1 Departamento de Bioquımica y Biologıa Molecular, Facultad de Ciencias Biologicas, Universitat de Valencia, Burjassot, Spain, 2 Departamento de Genetica, Facultad de ´ Biologıa, Universidad de Sevilla, Sevilla, Spain Abstract The amount of mRNA in a cell is the result of two opposite reactions: transcription and mRNA degradation. These reactions are governed by kinetics laws, and the most regulated step for many genes is the transcription rate. The transcription rate, which is assumed to be exercised mainly at the RNA polymerase recruitment level, can be calculated using the RNA polymerase densities determined either by run-on or immunoprecipitation using specific antibodies. The yeast Saccharomyces cerevisiae is the ideal model organism to generate a complete set of nascent transcription rates that will prove useful for many gene regulation studies. By combining genomic data from both the GRO (Genomic Run-on) and the RNA pol ChIP-on-chip methods we generated a new, more accurate nascent transcription rate dataset. By comparing this dataset with the indirect ones obtained from the mRNA stabilities and mRNA amount datasets, we are able to obtain biological information about posttranscriptional regulation processes and a genomic snapshot of the location of the active transcriptional machinery. We have obtained nascent transcription rates for 4,670 yeast genes. The median RNA polyme