a fiber-optic fluorescence microscope using a consumer-grade digital camera for in vivo cellular imaging一个光纤荧光显微镜使用体内细胞成像的消费级数码相机.pdfVIP

A Fiber-Optic Fluorescence Microscope Using a Consumer-Grade Digital Camera for In Vivo Cellular Imaging 1 1 2 3 Dongsuk Shin , Mark C. Pierce , Ann M. Gillenwater , Michelle D. Williams , Rebecca R. Richards- Kortum1* 1 Department of Bioengineering, Rice University, Houston, Texas, United States of America, 2 Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America, 3 Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America Abstract Background: Early detection is an essential component of cancer management. Unfortunately, visual examination can often be unreliable, and many settings lack the financial capital and infrastructure to operate PET, CT, and MRI systems. Moreover, the infrastructure and expense associated with surgical biopsy and microscopy are a challenge to establishing cancer screening/early detection programs in low-resource settings. Improvements in performance and declining costs have led to the availability of optoelectronic components, which can be used to develop low-cost diagnostic imaging devices for use at the point-of-care. Here, we demonstrate a fiber-optic fluorescence microscope using a consumer-grade camera for in vivo cellular imaging. Methods: The fiber-optic fluorescence microscope includes an LED light, an objective lens, a fiber-optic bundle, and a consumer-grade digital camera. The system was used to image an oral cancer cell line labeled with 0.01% proflavine. A human tissue specimen was imaged following surgical resection, enabling dysplastic and cancerous regions to be evaluated. The oral mucosa of a healthy