a novel miniature culture system to screen co2-sequestering microalgae一种新型微型屏幕co2-sequestering微藻的文化系统.pdfVIP

Energies 2012, 5, 4372-4389; doi:10.3390/en5114372 OPEN ACCESS energies ISSN 1996-1073 /journal/energies Article A Novel Miniature Culture System to Screen CO -Sequestering Microalgae 2 Wei Han 1, Chunying Li 1, Xiaoling Miao 1,2,* and Guangxin Yu 3 1 School of Life Science Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; E-Mails: xiaosanzao@ (W.H.); lichunying@ (C.L.) 2 State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China 3 CNOOC New Energy Investment Co. Ltd., Beijing 100016, China; E-Mail: yugx2@ * Author to whom correspondence should be addressed; E-Mail: miaoxiaoling@; Tel./Fax: +86-21 Received: 1 September 2012; in revised form: 23 October 2012 / Accepted: 23 October 2012 / Published: 1 November 2012 Abstract: In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO2 fixation. Cell growth under different CO2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L− 1 d− 1), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A samp