a role for the rna chaperone hfq in controlling adherent-invasive escherichia coli colonization and virulencerna的作用女伴hfq控制adherent-invasive大肠杆菌殖民和毒性.pdfVIP

A Role for the RNA Chaperone Hfq in Controlling Adherent-Invasive Escherichia coli Colonization and Virulence 1 1 1 2¤ 1 Karina T. Simonsen , Gorm Nielsen , Janni Vester Bjerrum , Thomas Kruse , Birgitte H. Kallipolitis , Jakob Møller-Jensen1* 1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark, 2 Novozymes, Bagsværd, Denmark Abstract Adherent-invasive Escherichia coli (AIEC) has been linked with the onset and perpetuation of inflammatory bowel diseases. The AIEC strain LF82 was originally isolated from an ileal biopsy from a patient with Crohn’s disease. The pathogenesis of LF82 results from its abnormal adherence to and subsequent invasion of the intestinal epithelium coupled with its ability to survive phagocytosis by macrophages once it has crossed the intestinal barrier. To gain further insight into AIEC pathogenesis we employed the nematode Caenorhabditis elegans as an in vivo infection model. We demonstrate that AIEC strain LF82 forms a persistent infection in C. elegans, thereby reducing the host lifespan significantly. This host killing phenotype was associated with massive bacterial colonization of the nematode intestine and damage to the intestinal epithelial surface. C. elegans killing was independent of known LF82 virulence determinants but was abolished by deletion of the LF82 hfq gene, which encodes an RNA chaperone involved in mediating posttranscriptional gene regulation by small non-coding RNAs. This finding reveals that important aspects of LF82 pathogenesis are controlled at the posttranscriptional level by riboregulation. The role of Hfq in LF82 virul