acetylation-mediated suppression of transcription-independent memory bidirectional modulation of memory by acetylationacetylation-mediated transcription-independent记忆双向调制的记忆的抑制乙酰化作用.pdfVIP

Acetylation-Mediated Suppression of Transcription- Independent Memory: Bidirectional Modulation of Memory by Acetylation 1 2 1 Katja Merschbaecher , Jakob Haettig , Uli Mueller * ¨ 1 Dept. 8.3 Biosciences Zoology/Physiology-Neurobiology, ZHMB (Center of Human and Molecular Biology), Saarland University, Saarbrucken, Germany, 2 Department of Neurobiology and Behavior, Center for the Neurobiology of Learning and Memory, University of California Irvine, Irvine, California, United States of America Abstract Learning induced changes in protein acetylation, mediated by histone acetyl transferases (HATs), and the antagonistic histone deacetylases (HDACs) play a critical role in memory formation. The status of histone acetylation affects the interaction between the transcription-complex and DNA and thus regulates transcription-dependent processes required for long-term memory (LTM). While the majority of studies report on the role of elevated acetylation in memory facilitation, we address the impact of both, increased and decreased acetylation on formation of appetitive olfactory memory in honeybees. We show that learning-induced changes in the acetylation of histone H3 at aminoacid-positions H3K9 and H3K18 exhibit distinct and different dynamics depending on the training strength. A strong training that induces LTM leads to an immediate increase in acetylation at H3K18 that stays elevated for hours. A weak training, not sufficient to trigger LTM, causes an initial increase in acetylation at H3K18, followed by a strong reduction in acetylation at H3K18 below the control group level. Acetylat