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alternative splicing regulated by butyrate in bovine epithelial cells可变剪接在牛上皮细胞由丁酸
Alternative Splicing Regulated by Butyrate in Bovine
Epithelial Cells
1 2 3 1 2
Sitao Wu , Congjun Li , Wen Huang , Weizhong Li , Robert W. Li *
1 Center for Research in Biological Systems, University of California San Diego, San Diego, California, United States of America, 2 USDA-ARS, Bovine Functional Genomics
Laboratory, Beltsville, Maryland, United States of America, 3 Department of Genetics, North Carolina State University, Raleigh, North Carolina, United States of America
Abstract
As a signaling molecule and an inhibitor of histone deacetylases (HDACs), butyrate exerts its impact on a broad range of
biological processes, such as apoptosis and cell proliferation, in addition to its critical role in energy metabolism in
ruminants. This study examined the effect of butyrate on alternative splicing in bovine epithelial cells using RNA-seq
technology. Junction reads account for 11.28 and 12.32% of total mapped reads between the butyrate-treated (BT) and
control (CT) groups. 201,326 potential splicing junctions detected were supported by $3 junction reads. Approximately
94% of these junctions conformed to the consensus sequence (GT/AG) while ,3% were GC/AG junctions. No AT/AC
junctions were observed. A total of 2,834 exon skipping events, supported by a minimum of 3 junction reads, were
detected. At least 7 genes, their mRNA expression significantly affected by butyrate, also had exon skipping events
differentially regulated by butyrate. Furthermore, COL5A3, which was induced 310-fold by butyrate (FDR ,0.001) at the
gene level, had a significantly higher number of junction reads mapped to Exon#8 (Donor) and Exon#11 (Acceptor) in BT.
This event had the potential to res
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