antifibrinolytic role of a bee venom serine protease inhibitor that acts as a plasmin inhibitor纤溶作用的蜂毒丝氨酸蛋白酶抑制剂,作为血纤维蛋白溶酶抑制剂.pdfVIP

Antifibrinolytic Role of a Bee Venom Serine Protease Inhibitor That Acts as a Plasmin Inhibitor 1. 1. 2 1 1 1 Young Moo Choo , Kwang Sik Lee , Hyung Joo Yoon , Yuling Qiu , Hu Wan , Mi Ri Sohn , 1 1 Hung Dae Sohn , Byung Rae Jin * 1 College of Natural Resources and Life Science, Dong-A University, Busan, Republic of Korea, 2 Department of Agricultural Biology, National Academy of Agricultural Science, Suwon, Republic of Korea Abstract Bee venom is a rich source of pharmacologically active substances. In this study, we identified a bumblebee (Bombus ignitus) venom Kunitz-type serine protease inhibitor (Bi-KTI) that acts as a plasmin inhibitor. Bi-KTI showed no detectable inhibitory effect on factor Xa, thrombin, or tissue plasminogen activator. In contrast, Bi-KTI strongly inhibited plasmin, indicating that it acts as an antifibrinolytic agent; however, this inhibitory ability was two-fold weaker than that of aprotinin. The fibrin(ogen)olytic activities of B. ignitus venom serine protease (Bi-VSP) and plasmin in the presence of Bi-KTI indicate that Bi- KTI targets plasmin more specifically than Bi-VSP. These findings demonstrate a novel mechanism by which bumblebee venom affects the hemostatic system through the antifibrinolytic activity of Bi-KTI and through Bi-VSP-mediated fibrin(ogen)olytic activities, raising interest in Bi-KTI and Bi-VSP as potential clinical agents. Citation: Choo YM, Lee KS, Yoon HJ, Qiu Y, Wan H, et al. (2012) Antifibrinolytic Role of a Bee Venom Serine Protease Inhibitor That Acts as a Plasmin Inhibitor. PLoS ONE 7(2): e32269. doi:10.1371/journal.pone