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application of oligonucleotide microarrays for bacterial source tracking of environmental enterococcus sp. isolates应用寡核苷酸微阵列环境肠球菌细菌源跟踪sp,隔离
Int. J. Environ. Res. Public Health 2005, 2(1), 175–185
International Journal of
Environmental Research and Public Health
ISSN 1660-4601
© 2005 by MDPI
Application of Oligonucleotide Microarrays for Bacterial Source
Tracking of Environmental Enterococcus sp. Isolates.
1* 3 2
Karl J. Indest , Kelley Betts , and John S. Furey
1U.S. Army Engineer Research and Development Center, Waterways Experiment Station, 3909 Halls Ferry Road,
Vicksburg, MS 39180, USA, 2CSC, 3530 Manor Drive, Vicksburg, MS 39180, 3Analytical Services, Inc., 555 Sparkman
Drive, Huntsville, Alabama 35816, USA
*Correspondence to Dr. Karl J. Indest. E-mail: indestk@
Received: 15 November 2004 / Accepted: 06 February 2005 / Published: 30 April 2005
Abstract: In an effort towards adapting new and defensible methods for assessing and managing the risk posed
by microbial pollution, we evaluated the utility of oligonucleotide microarrays for bacterial source tracking (BST)
of environmental Enterococcus sp. isolates derived from various host sources. Current bacterial source tracking
approaches rely on various phenotypic and genotypic methods to identify sources of bacterial contamination
resulting from point or non-point pollution. For this study Enterococcus sp. isolates originating from deer,
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