GFAP与端粒酶在衰老痴呆大鼠脑组织中的表达.docVIP

GFAP与端粒酶在衰老痴呆大鼠脑组织中的表达 蔡志友1晏勇1，余昌胤2，张骏2，黄良国2，杨丽2，詹剑2，王咏龙1，李杰颖1，吴芳1 (1重庆医科大学附属第一医院神经内科 重庆市神经病学重点实验室，重庆 400016； 2遵义医学院附属医院神经内科，贵州遵义563003） 摘要：目的 观察GFAP与端粒酶表达，探讨衰老与老年痴呆的关系及作用机制。方法 Wistar随机分：正常组（N组）、（组）组（组）（组）。GFAP在衰老痴呆大鼠脑组织中的表达，图像分析仪测定光密度值。结果(P0.01)，GFAP与端粒酶在模型组和依达拉奉治疗组表达明显增高 (P0.01)；模型组与依达拉奉治疗组比较依达拉奉治疗组GFAP表达显著下降(P0.01)，端粒酶表达明显增高(P0.05)，行为学症状显著改善(P0.01)。结论关键词；Expressin of GFAP and telomerase in brain with aged dementia rats Zhiyou Cai1,Yong Yan1, Changyin YU3, Jun Zhang3, Huangliang GUO3, Li YANG3, Jian ZHAN3, Longyong WANG1,Jieying LI, Fang Wu1 (1:Neurology Department , the First Affiliated Hospital of Chongqing Medical University 400016; 2: Chongqing Medical University 400016; 3:Neurology Department of the Affiliated Hospital of Zunyi Medical College 563003）ABSTRACT：Objective Tophysiological mechanism of GFAP and telomerase for aged dementia by investigating expression of GFAP and telomerase for aged dementia in Wistar rats. Mthethods Wistar rats were randomly divided into: normal groups(N), sham groups(S)、vascular dementia model groups(M) and edaravone treatment groups(MT). The level of SOD and MDA was measured by biochemistry. Expression of telomerase was measured by immunohistochemistry and by enzyme linked immunosorbent assay and GFAP by immunohistochemistry, western blotting and by enzyme linked immunosorbent assay .The light density value was measured by imaging analysis. Results The expression of GFAP and telomerase in MT and M groups is higher than that of in N, S groups(P0.05). The expression of telomerase in MT group is significantly higher than that of in M (P0.01). The expression of GFAP in MT group is significantly lower than that of in M (P0.01).Conclusions Oxidative stress, reactivated astrocyte and chronic neuro-inflammation contribute to aging and dementia. Telomerase can enhance cell proliferation to protect neural function and restrain aging. Key words aged dementia, glial fibrillary acidic protein, telomerase 自由基损伤参与了衰老过程。随年龄的增长，人体内自由基水平呈增长趋