HPLC法测定射干配方颗粒中射干苷的含量.PDFVIP

hin J Pharm Anal2009, 29( 8) # 1375 # HPLC 郭志辉 (, 300052) : : , D iamonsil 18 ( 46mm 250mm, 5 m) - 1 , - 02% ( 78 22), 10 mL! m in , 265 nm, 35 ∀ : 006~ 048g, r= 09999 3( n = 3) 9905% , 1001% , 1000% ; RSD 051% , 14% , 055% : , , : ; ; : R917 : A : 0254- 1793( 2009) 08- 1375- 03 HPLC determ ination of belam candin in Shegan dispensing granules GUO Zhi- hui ( T ianjin entral of Obstetrics and GynecologyHospital, T ianjin 300052, hina) Abstract Objective: To determ ine the content of belamcandinin Shegan dispensing granules.M ethods: TheHPL method was established. The analysiswas carried out onD iamonsil 18 ( 46mm 250mm, 5 m) column; Themo - 1 bile phase of belamcandinwas acetonitrile- 02% phosphoric acid(78 22) at a flow rate of 10mL!m in ; The detection wavelength was 265 nm and the column temperaturewas35 ∀ . Results: The linear range of belam candin was 006- 048 g( r = 09999). In the high, m edium, low concentration, the average recoveries of belam candin were 9905%, 1001%, 1000% w ith corresponding RSD 051%, 143% , 055% . Conclusion: TheHPL m eth od is easy and precise to determ ine the content of belamcandinin Shegan dispensing granules. K e words: Shegan dispensing granules; belamcandin; HPL [B elam canda ch inensis ( L. ) D . ], 1 , Agilent 1100, Agilent 1100 , , Agilent (, : 111632- 200501); , , , (,