Irbesartan对糖尿病大鼠肾组织骨桥蛋白的调节.docVIP

Irbesartan对糖尿病大鼠肾组织骨桥蛋白的调节 肖柯，曹文富，焦颖华，陈益山（重庆医科大学附属第一医院中西医结合科，重庆 400016） 摘要：目的 观察血管紧张素Ⅱ(AngⅡ)受体拮抗剂Irbesartan对糖尿病 (DM)大鼠肾组织骨桥蛋白表达及炎症细胞浸润的影响。 方法 建立STZ诱导的糖尿病SD大鼠模型，将糖尿病大鼠随机分成组、Irbesartan组，同时设正常对照组。UAER)，HE染色观察大鼠肾脏病理形态变化，免疫组化法检测肾小管骨桥蛋白（OPN），CD68表达，Western?bloting?检测OPN在肾中的蛋白表达。结果 DM模型组UAER、尿素氮、血肌酐、肾指数显著增高；肾组织OPN，CD68表达明显增多，光镜下肾小管结构异常改变明显，Irbesartan组组(P001)，肾脏超微结构明显改善，但未达到正常对照组 R972+.4;R587.2 文献标识码:A Effect of irbesartan on activation of renal osteopontin in diabetic rats XIAO ke, CAO Wenfu, JIAO yinhua，CHEN Yishan (Department of Traditional Chinese Medicine Integrated with Western Medicine，First Affiliated Hospital of Chongqing Medical University，Chongqing 400016) Objective To investigate the effect of AT1 receptor antagonist irbesartan on expression of renal osteopontin in diabetic rats and influence of infiltration of inflammatory cell. Methods The diabetic SD rats induced by STZ were divided into two groups: diabetic control group and Irbesartan group, and the normal control group. After experimental rats in every group were respectively treated for 12 weeks by above intervention methods. The relative kidney weight, blood sugar, urea nitrogen, serum creatinine, and urinary albumin excretion rate (UAER) were detected by routine analysis methods. The pathological changes of the rat kidney were also observed by HE staining. Exprssion of osteopontin(OPN) and macrophages antigen (CD68) in renal tubule were determined by immunohistochemical technique, and OPN in renal was detected by Western blot methods．Results The blood sugar, UAER, urea nitrogen, serum creatinine, relative kidney weight were significantly increased in diabetic rats. Exprssion of OPN and CD68 were significantly increased, the tubular structure changed significantly under light microscope. Irbesartan group were significantly lower than the diabetic control group (P0.01), and microstructure of kidney was obviously improved, not yet restore to the level of the normal control group. Conclusion Irbe