OPGRANKLRANK系统参与牙槽骨吸收及重建过程作用的初探.docVIP

OPG/RANKL/RANK系统参与牙槽骨吸收及重建过程作用的初探 （ 310009杭州浙江大学医学院附属第二医院口腔内科）摘要目的 初步探讨护骨素OPG核因子κB受体活化因子配体RANKL核因子κB活化因子RANK在牙周炎牙槽骨吸收及骨重建过程中的作用。方法 对小鼠RAW264.7实验组进行体外诱导培养小鼠MC3T3-E1实验组采用前期实验收集的骨吸收上清处理，以免疫荧光等方法检测细胞OPGRANKL、RANK的表达。大鼠建立实验性牙槽骨吸收模型，研究其吸收重建规律。以免疫组化S-P法检测OPGRANKL、RANK的表达情况。结果MC3T3-E细胞经骨吸收上清OPG蛋白29.636±5.652，对照组为15.568±1.229，表达显著上调（P0.01）但RANKL蛋白6.806±1.738，对照组为18.082±2.732表达被显著抑制P0.01），OPGRANKL比值在上清后高于对照组P0.05）。采用大肠杆菌内毒素E-LPS注射法成功制备大鼠牙槽骨吸收模型。在牙槽骨吸收区域OPG水平较有所降低，RANKL的表达。结论OPG、RANKL、RANK参与了大鼠实验性骨吸收关键词成骨细胞；破骨细胞；护骨素； 核因子κB受体活化因子配体；核因子κB活化因子；牙周炎动物模型中图法分类号OPG/RANKL/RANK system during alveolar bone resorption and remodeling Chen Lili, Huang Mei, Lei Lihong, Wu Yanming (Department of Oral Medicine, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, 310009, China）Abstract] Objective To investigate the potential role of a system containing osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL), and receptor activator of nuclear factor-kappa B (RANK), also as, OPG/RANKL/RANK system in the resorption and remodeling mechanisms of alveolar bone. Methods Mouse RAW264.7 cell line was induced to osteoclasts in vitro, and mouse MC3T3-E1 cell line was treated with bone resorption supernatant. The control cells was cultured with regular medium. The expression levels of OPG, RANKL and RANK in the cells was detected by immunofluorescence assay. Thirty 8-week-old male SD rats were used to establish the alveolar bone resorption model with the injection of E-LPS. Immunohistochemical S-P method was used to investigate the expression of OPG, RANKL and RANK in the animal model. Results The expression of OPG in MC3T3-E1 cells was up-regulated after 7 days’ treatment with bone resorption supernatant than in control cells (29.636±5.652 vs 15.568±1.229, P0.01）, while, the RANKL expression was down-regulated （6.806±1.738 vs 18.082±2.732, P0.01）. The ratio of OPG to RANKL was up-regulated too （P0.05）. The OPG level in periodontal tissues of rat