RanBP9的表达对骨肉瘤细胞增殖,凋亡及转移的影响.docVIP

RanBP9的表达对骨肉瘤细胞增殖、凋亡及转移的影响﹡ 张樨1，吕杨帆1， 戴欢子2，孟刚3，郭乔楠1 （重庆，第三军医大学新桥医院病理科1第三军医大学大坪医院肾内科2第三军医大学西南医院病理科3） 【摘要】 RanBP9（Ran-binding protein 9）在骨肉瘤细胞的表达情况和对骨肉瘤细胞增殖、凋亡及转移的影响，RanBP9在骨肉瘤细胞生物学功能中发挥的作用。方法 Real-time PCR、免疫荧光细胞染色和Western-blot检测不同骨肉瘤RanBP9的mRNA和蛋白表达水平；利用慢病毒建立稳定低表达RanBP9的SaOS2细胞模型并检测低表达RanBP9对骨肉瘤细胞SaOS2细胞增殖、克隆形成、凋亡及迁移、侵袭等细胞生物学功能的影响。RanBP9在MG63、SaOS2、U2OS三个骨肉瘤细胞系中在低级别骨肉瘤细胞系MG63中的表达高于高级别细胞系SaOS2和U2OS（P0.05）稳定低表达RanBP9的SaOS2细胞（siRanBP9）细胞增殖、克隆形成、迁移、侵袭能力均较对照细胞siCON-SaOS2（siCON）增强，差异有统计学意义（P0.05），而凋亡率下降，有显著差异（P0.05）。RanBP9的低表达了骨肉瘤细胞的增殖和转移能力，了骨肉瘤细胞的凋亡, RanBP9可能作为一个抑癌基因参与了骨肉瘤的发生与演进。 【关键词】 RanBP9The effect of RanBP9 on proliferation，apoptosis and metastasis in human osteosarcoma cells Zhang Xi1,Lv Yangfan1,Dai Huanzi2,Meng Gang3,Guo Qiaonan1 (1Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing ,400037, China; 2 Department of Nephrology ,Daping Hospital, Third Military Medical University, Chongqing, 400042, China; 3 Department of Pathology, Southwest Hospital, Third Military Medical University, Chongqing ,400038, China) 【Abstract】 Objective To explore the effect of on proliferation，apoptosis and metastasis in human osteosarcoma, so as to discuss its biology functions in osteosarcoma cells. Methods Real-time PCR, immunofluorescence and Western-blot to examine RanBP9 mRNA and protein expression levels in osteosarcoma cell lines. Also we performed a series of biological tests using in osteosarcoma SaOS2 cell line with down-regulated RanBP9. Results We found that RanBP9 is highly expressed in human osteosarcoma cell lines and RanBP9 expression in MG63 cell line derived from low-grade osteosarcoma was significant higher (p0.05) than in U2OS or SaOS2 cell lines derived from high-grade osteosarcoma. Immunofluorescence and Western-blot also showed the same result as the Real-time PCR. Low expression of RanBP9 in SaOS2 cell line not only markedly increased cell vitality and growth, colony formation, but also stimulated migration and invasio