PTEN,Akt,TGF-β1蛋白在瘢痕疙瘩中的表达.docVIP

PTEN,Akt,TGF-β1蛋白在瘢痕疙瘩中的表达，徐凯，章鑫，黄巧玲，桂思，晏洪波，李世荣 广州军区武汉总医院皮肤整形美容外科 湖北 武汉 430070 【摘　要】目的探讨瘢痕疙瘩组织中TGF-β1、第十号染色体同源丢失性磷酸酶基因（phosphatase and tensin homolog deleted on Chromosome 10，PTEN)和蛋白激酶（Akt）的表达。方法采用免疫组化SP法检测TGF-β1PTEN和p-Akt在2例瘢痕疙瘩20例正常皮肤组织中的表达水平。结果瘢痕疙瘩组织中，TGF-β1, Akt蛋白阳性表达率分别为4.94±7.59以及44.95±6.65，均高于正常皮肤组（P0. 01）；PTEN蛋白阳性表达率为.53±1.45，低于组(P0. 01)。PTEN的表达与Akt, TGF-β1的表达强度成负相关(r0.6437,-0.376)，TGF-β1与Akt表达成正相关（r=0. ）。结论PTEN，Akt及TGF-β1在瘢痕疙瘩的发生发展中均起着重要的调控作用，PTEN/Akt信号传导通路可能通过调控TGF-β1参与瘢痕的形成。TGF-β1 Expressions of PTEN,Akt and TGF-β1 protein in keloid Guo Liang, Xu Kai, Zang Xin, Huang Qiaoling, Gui Si, Yan hongbo, Li Shirong (Department Of Plastic Surgery, Wuhan General Hospital of Guangzhou Military Region, Wuhan,430070,Hubei, China) Abstract: Objective: To investigate the expressions of TGF-β1, p-Akt and PTEN proteins in keloid. Methods: SP immunohistochemical method was used to detect the expressions of TGF-β1, p-Akt and PTEN proteins in 28 cases of pathologic scar and 20 cases of normal skin tissue. Results: The positive rates of TGF-β1 and p-Akt proteins in 28 cases of keloid were43.94±7.59 and 44.95±6.65, were higher respectively compared with the control groups (P0.01);The rate of PTEN proteins in keloid group was 6.53±1.45,significantly lower than the normal skin group (P 0.01) .A negative correlation was found between the expression of PTEN and p-Akt or TGF-β1 proteins (r=-0.6437,-0.376) in keloid, and there was a highly positive correlation between TGF-β1 and p-Akt protein expression (r=0. 804). Conclusion: PTEN，Akt and TGF-β1 protein might be involved in the formation of keloid. PTEN/Akt signaling pathway may regulate the information of keloid through the activation of TGF-β1. Key words：TGF-β1 示. 05, r 瘢痕疙瘩（Keloid）是皮肤组织在发生创伤、烧伤或手术后引发一种疾病，其病理特征是成纤维细胞过度增生和细胞外基质的异常积聚其中成纤维细胞的增殖/凋亡调控失调是其产生的重要原因之一，临床表现为侵犯临近组织呈瘤样生长，质硬，伴有不同程度的瘙痒和疼痛，常造成功能障碍和外形破坏。国内外许多学者对