丙酮酸乙酯对烫伤大鼠肺组织高迁移率蛋白B1表达和肺损伤的影响.docVIP

丙酮酸乙酯对烫伤大鼠肺组织高迁移率蛋白B1 表达和肺损伤的影响 王彦博1，姚咏明王 强王文江刘玉峰1 1第四军医大学西京医院皮肤科西安 710032；2解放军总医院第一附属医院烧伤研究所，北京 100037；3解放军总医院第二附属医院普外科，北京 100091） 摘要目的 观察丙酮酸乙酯EP）对烫伤大鼠肺组织高迁移率蛋白B1HMGB1）表达水平及急性肺损的 方法 采用大鼠30%总体表面积Ⅲ度烫伤模型，78只雄性Wistar大鼠随机分为假烫伤组（n=18）烫伤组（n=30）EP治疗组（n=30），每组分别在假伤或伤后第8、24、72活杀留取肺组织。HMGB1基因/蛋白表达分别采用逆转录聚合酶链反应及蛋白免疫印记法免疫组化方法检测髓过氧化物酶MPO）活性采用酶学分光光度法测定并采用HE染色、光镜下观察肺组织病理改变。结果 与假烫伤组比较，烫伤组肺组织HMGB1基因/蛋白表达于伤后8~72 h显著增强P 0.05或P 0.01，同时组织MPO活性在8 h及24 h明显高（P 0.01）病理学观察见肺组织炎细胞浸润，正常结构破坏其中以24 h最重。与烫伤组比较，治疗组大鼠肺组织872 h时间点HMGB1表达显著下调P 0.05），肺组织MPO活性在8 h、24 h时间点显著降P 0.01），治疗组872 h肺组织病理形态明显减轻。结论 HMGB1作为晚期炎症因子参与了烫伤后肺组织炎症反应的病理过程，治疗可明显下调肺组织HMGB1表达，并有助于降低肺组织MPO活性，从而减轻烫伤延迟复苏所致急性肺损伤。 基金项目：国家重点基础研究发展计划（973计划）2005CB522602）；国家自然科学基金 Supported by the National Basic Research Program (973 Program) (2005CB522602) and the National Natural Science Foundation of China 作者简介：王彦博，女，人，博士研究生，主治医师，主要从事免疫研究。通信作者：姚咏明，电话：(010 E-mail: c_ff@ Effect of ethyl pyruvate on pulmonary high mobility group box-1 protein expression in rats with delayed resuscitation after burn injury WANG Yan-bo1, YAO Yong-ming2, WANG Qiang, WANG Wen-jiang2, LIU Yu-feng1 （1Department of Dermatology, Xjiing Hospital, Fourth Military Medical University, Xi’an 710032，China Abstract：Objective To investigate the effect of ethyl pyruvate (EP) on pulmonary high mobility group box-1 protein (HMGB1) expression and acute lung injury in rats with delayed resuscitation after burn injury. Methods Wistar rats were subjected to 30% full-thickness scald injury followed with delayed resuscitation. 78 male rats were randomly divided into sham scald group (n=18), scald group (n=30), and EP treatment group (n=30), and they were sacrificed at 8h, 24h, 72h after scald injury, respectively. Lung tissue samples were harvested to determine HMGB1 mRNA as well as protein expressions, and pulmonary myeloperoxidase (MPO) activity. HMGB1 mRNA level was semi-quantitatively measured by the reverse transcription polymerase chain rea