quantitative proteomics identifies the myb-binding protein p160 as a novel target of the von hippel-lindau tumor suppressor定量蛋白质组学鉴定myb-binding蛋白p160冯hippel-lindau肿瘤抑制的一种新颖的目标.pdfVIP

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quantitative proteomics identifies the myb-binding protein p160 as a novel target of the von hippel-lindau tumor suppressor定量蛋白质组学鉴定myb-binding蛋白p160冯hippel-lindau肿瘤抑制的一种新颖的目标.pdf

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quantitative proteomics identifies the myb-binding protein p160 as a novel target of the von hippel-lindau tumor suppressor定量蛋白质组学鉴定myb-binding蛋白p160冯hippel-lindau肿瘤抑制的一种新颖的目标

Quantitative Proteomics Identifies the Myb-Binding Protein p160 as a Novel Target of the von Hippel-Lindau Tumor Suppressor 1. 1. 1 2 1,2 Yanlai Lai , Mei Qiao , Meihua Song , Susan T. Weintraub , Yuzuru Shiio * 1 Greehey Children’s Cancer Research Institute, San Antonio, Texas, United States of America, 2 Department of Biochemistry, The University of Texas Health Science Center, San Antonio, Texas, United States of America Abstract Background: The von Hippel-Lindau (VHL) tumor suppressor gene encodes a component of a ubiquitin ligase complex, which is best understood as a negative regulator of hypoxia inducible factor (HIF). VHL ubiquitinates and degrades the a subunits of HIF, and this is proposed to suppress tumorigenesis and tumor angiogenesis. However, several lines of evidence suggest that there are unidentified substrates or targets for VHL that play important roles in tumor suppression. Methodology/Principal Findings: Employing quantitative proteomics, we developed an approach to systematically identify the substrates of ubiquitin ligases and using this method, we identified the Myb-binding protein p160 as a novel substrate of VHL. Conclusions/Significance: A major barrier to understanding the functions of ubiquitin ligases has been the difficulty in pinpointing their ubiquitination substrates. The quantitative proteomics approach we devised for the identification of VHL substrates will be widely applicable to other ubiquitin ligases. Citation: Lai Y, Qiao M, Song M, Weintraub ST, Shiio Y (2011) Quantitative Proteomics Identifies the Myb-Binding Protein p160 as a Novel Target of the von Hippel-Lindau Tumor Suppressor. PLoS ONE 6(2): e16975. doi:10.1371/journal.pone.0016975 Editor: