rab11a-controlled assembly of the inner membrane complex is required for completion of apicomplexan cytokinesisrab11a-controlled组装膜内部的复杂需要完成apicomplexan胞质分裂.pdfVIP

Rab11A-Controlled Assembly of the Inner Membrane Complex Is Required for Completion of Apicomplexan Cytokinesis 1. 2. 2 1 Carolina Agop-Nersesian , Bernina Naissant , Fathia Ben Rached , Manuel Rauch , Angelika 1 3 3 4 1 Kretzschmar , Sabine Thiberge , Robert Menard , David J. P. Ferguson , Markus Meissner *, Gordon Langsley2* 1 Hygieneinstitut, Department of Parasitology, University Hospital Heidelberg, Heidelberg, Germany, 2 Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, ´ ´ ˆ ´ Department of Infectious Diseases, Institut Cochin, Inserm U567, CNRS UMR 8104, Faculte de Medecine Paris V – Hopital Cochin, Paris, France, 3 Unite de Biologie et ´ ´ Genetique du Paludisme, Institut Pasteur, Paris, France, 4 Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom Abstract The final step during cell division is the separation of daughter cells, a process that requires the coordinated delivery and assembly of new membrane to the cleavage furrow. While most eukaryotic cells replicate by binary fission, replication of apicomplexan parasites involves the assembly of daughters (merozoites/tachyzoites) within the mother cell, using the so- called Inner Membrane Complex (IMC) as a scaffold. After de novo synthesis of the IMC and biogenesis or segregation of new organelles, daughters bud out of the mother cell to invade new host cells. Here, we demonstrate that the final step in parasite cell division involves delivery of ne