原创力文档rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis using a multiplex real-time pcr assay快速鉴别诊断肺外结核病和焦之间使用多路实时pcr检测布鲁氏菌病的并发症.pdfVIP
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rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis using a multiplex real-time pcr assay快速鉴别诊断肺外结核病和焦之间使用多路实时pcr检测布鲁氏菌病的并发症
Rapid Differential Diagnosis between Extrapulmonary
Tuberculosis and Focal Complications of Brucellosis
Using a Multiplex Real-Time PCR Assay
´ ˜ 1,2 3 4 ´ ´ 5 1,2
Marıa Isabel Queipo-Ortuno *, Juan D. Colmenero , Pilar Bermudez , Marıa Jose Bravo , Pilar Morata
´ ´
1 Biochemistry and Molecular Biology Department, Faculty of Medicine, University of Malaga, Malaga, Spain, 2 CIBER Fisiopatologıa Obesidad y Nutricion (CB06/03)
Instituto de Salud Carlos III, Madrid, Spain, 3 Infectious Diseases Service, Carlos Haya University Hospital, Malaga, Spain, 4 Microbiology Service, Carlos Haya University
Hospital, Malaga, Spain, 5 Immunology Service, Carlos Haya University Hospital, Malaga, Spain
Abstract
Background: Arduous to differ clinically, extrapulmonary tuberculosis and focal complications of brucellosis remain
important causes of morbidity and mortality in many countries. We developed and applied a multiplex real-time PCR assay
(M RT-PCR) for the simultaneous detection of Mycobacterium tuberculosis complex and Brucella spp.
Methodology: Conventional microbiological techniques and M RT-PCR for M. tuberculosis complex and Brucella spp were
performed on 45 clinical specimens from patients with focal complications of brucellosis or extrapulmonary tuberculosis
and 26 control samples. Fragments of 207 bp and 164 bp from the conserved region of the genes coding for an
immunogenic membrane protein of 31 kDa of B. abortus (BCSP31) and the intergenic region SenX3-Reg
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