rates of mutation and host transmission for an escherichia coli clone over 3 years率的突变和主机传输大肠杆菌克隆3年以上.pdfVIP

Rates of Mutation and Host Transmission for an Escherichia coli Clone over 3 Years 1 2,3 2,3 2,3 2,3 2,3 4 Peter R. Reeves , Bin Liu , Zhemin Zhou , Dan Li , Dan Guo , Yan Ren , Connie Clabots , Ruiting 5 4 2,3,6 Lan , James R. Johnson , Lei Wang * 1 School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia, 2 Tianjin Economic-Technological Development Area School of Biological Sciences and Biotechnology, Nankai University, Tianjin, China, 3 The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China, 4 Veterans Affairs Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota, United States of America, 5 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia, 6 Tianjin Research Center for Functional Genomics and Biochip, Tianjin, China Abstract Although over 50 complete Escherichia coli/Shigella genome sequences are available, it is only for closely related strains, for example the O55:H7 and O157:H7 clones of E. coli, that we can assign differences to individual evolutionary events along specific lineages. Here we sequence the genomes of 14 isolates of a uropathogenic E. coli clone that persisted for 3 years within a household, including a dog, causing a urinary tract infection (UTI) in the dog after 2 years. The 20 mutations observed fit a single tree that allows us to estimate the mutation rate to be about 1.1 per genome per year, with minimal evidence for adaptive change, including in relation to the UTI episode. The host data also imply at least 6 host tr