reassortant h9n2 influenza viruses containing h5n1-like pb1 genes isolated from black-billed magpies in southern china重组h9n2流感病毒包含类似h5n1的pb1基因隔绝black-billed喜鹊在中国南部.pdfVIP

Reassortant H9N2 Influenza Viruses Containing H5N1- Like PB1 Genes Isolated from Black-Billed Magpies in Southern China 1,2. 3. 1 1 1 1 4 Guoying Dong , Cong Xu , Chengmin Wang , Bin Wu , Jing Luo , Hong Zhang , Dale Louis Nolte , 4 5 2 1 Thomas Jude Deliberto , Mingxing Duan , Guangju Ji *, Hongxuan He * 1 Key Laboratory of Animal Ecology and Conservation Biology, National Research Center For Wildlife Born Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, 2 National Laboratory of Biomacromolecules, Institute of Biophysics of Chinese Academy of Sciences, Beijing, China, 3 College of Food Science, Southwest University, Chongqing, China, 4 National Wildlife Disease Program, USDA/APHIS/Wildlife Services, United States Department of Agriculture, Fort Collins, Colorado, United States of America, 5 State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing, China Abstract H9N2 influenza A viruses have become endemic in different types of terrestrial poultry and wild birds in Asia, and are occasionally transmitted to humans and pigs. To evaluate the role of black-billed magpies (Pica pica) in the evolution of influenza A virus, we conducted two epidemic surveys on avian influenza viruses in wild black-billed magpies in Guangxi, China in 2005 and characterized three isolated black-billed magpie H9N2 viruses (BbM viruses). Phylogenetic analysis indicated that three BbM viruses were almost identical with 99.7 to 100% nucleotide homology in their whole genomes, and were reassortants containing BJ94-like (Ck/BJ/1/94) HA, NA, M, and N