regarding the amazing choreography of clathrin coats关于网格蛋白外套的惊人的编排.pdfVIP

Primer Regarding the Amazing Choreography of Clathrin Coats Linton M. Traub* Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America The growth of contemporary cell biology is due in large part to At least 40 different proteins participate in the construction of a technological advances. In the 1950s, electron micrographs of thin clathrin-coated endocytic vesicle [1,5,6]. Precisely when and how sections first provided unrivaled in situ views of the delicate the many distinct proteins interact as the vesicle forms, how intracellular architecture and fine structure of organelles, whereas information is relayed, and how directionality is assured without new subcellular fractionation methods gave access to various malfunction, given there is no obvious coupled input of energy to biochemical components—especially proteins—enriched in differ- instigate budding, is currently uncertain. Also, it remains possible ent cellular fractions. A central tenet that emerged from these that various combinations of these many factors might build pioneering studies is that the intracellular biosynthetic and structurally distinct sorting structures, perhaps associated with endocytic membrane systems of eukaryotic cells are functionally separable functions [7–10]. Some empirical evidence for this interconnected, and exchange of material between them often actually exists: electron micrographs show both isolated ,100-nm occurs in small (50–100 nm diameter), roughly spherical mem- diameter clathrin-coated buds as well as large expanses of