角质蛋白Keratin-15及胃癌浸润转移的相关性研究.pdf

目 录 中文摘要······················································································1 英文摘要······················································································2 缩写词表······················································································3 引 言······················································································4 材料和方法···················································································6 1、临床标本············································································6 2、细胞株和菌株及主要质粒····················································6 3、主要仪器············································································6 4、主要试剂············································································7 5、常用溶液配制·····································································8 6、实验方法············································································9 结果····························································································22 1、免疫组化结果判定及相关分析············································22 2、Keratin15 基因片段扩增及重组质粒的酶切鉴定·················24 3、Keratin15 重组质粒表达情况············································25 4、EMT 相关蛋白表达情况·······················································26 5、划痕实验比较细胞迁移运动能力········································26 6、Transwell 小室检测细胞迁移能力·····································27 讨论····························································································29 1、利用免疫组化技术分析keratin15 在胃癌组织中表达情况··29 2、Keratin15 胃癌浸润、转移的关系 „„„„„„„„„„„„30 结 论·····················································································31 参考文献·····················································································32 致 谢·····················································································