sequencing by hybridization of long targets杂交测序的长期目标.pdfVIP

Sequencing by Hybridization of Long Targets Yu Qin, Tobias M. Schneider, Michael P. Brenner* School of Engineering and Applied Sciences and Kavli Institute for Bionano Science and Technology, Harvard University, Cambridge, Massachusetts, United States of America Abstract Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n ~O(2l ) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error. Citation: Qin Y, Schneider TM, Brenner MP (2012) Sequencing by Hybridization of Long Targets. PLoS ONE 7(5): e35819. doi:10.1371/journal.pone.0035819 Editor: Cynthia Gibas, University of North Carolina at Charlotte, United States of America Received September 15, 2011; Accepted March 26, 2012; Published May 4, 2012 Copyright: 2012 Qin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work is supported by the National Science Foundation, through the Harvard Materials Research and Engineering Center DMR-0820484) and the Division of Mathematical Sciences (DMS-0907985). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: brenner@ Introduction i.e. n