sequencing, mapping, and analysis of 27,455 maize full-length cdnas测序、映射和分析27455年玉米长篇的互补.pdfVIP

Sequencing, Mapping, and Analysis of 27,455 Maize Full-Length cDNAs 1 1 2 1 1 2 Carol Soderlund *, Anne Descour , Dave Kudrna , Matthew Bomhoff , Lomax Boyd , Jennifer Currie , 2 2 2 2 3 Angelina Angelova , Kristi Collura , Marina Wissotski , Elizabeth Ashley , Darren Morrow , John 3 3 2 Fernandes , Virginia Walbot , Yeisoo Yu * 1 BIO5 Institute, University of Arizona, Tucson, Arizona, United States of America, 2 Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, Arizona, United States of America, 3 Department of Biology, Stanford University, Stanford, California, United States of America Abstract Full-length cDNA (FLcDNA) sequencing establishes the precise primary structure of individual gene transcripts. From two libraries representing 27 B73 tissues and abiotic stress treatments, 27,455 high-quality FLcDNAs were sequenced. The average transcript length was 1.44 kb including 218 bases and 321 bases of 59 and 39 UTR, respectively, with 8.6% of the FLcDNAs encoding predicted proteins of fewer than 100 amino acids. Approximately 94% of the FLcDNAs were stringently mapped to the maize genome. Although nearly two-thirds of this genome is composed of transposable elements (TEs), only 5.6% of the FLcDNAs contained TE sequences in coding or UTR regions. Approximately 7.2% of the FLcDNAs are putative transcription factors, suggesting that rare transcripts are well-enriched in our FLcDNA set. Protein similarity searching identified 1,737 maize transcripts not present in rice