significance of mir-196b in tumor-related epilepsy of patients with gliomasmir - 196 b的意义在肿瘤癫痫患者的神经胶质瘤.pdfVIP

Significance of miR-196b in Tumor-Related Epilepsy of Patients with Gliomas 1. 1. 1 1 1 3 4 5 Gan You , Wei Yan , Wei Zhang , Yongzhi Wang , Zhaoshi Bao , Shouwei Li , Shaowu Li , Guilin Li , 2 2 1,3 Yijun Song , Chunsheng Kang , Tao Jiang * 1 Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China, 2 Department of Neurosurgery, Tianjin Medical University General Hospital, Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, China, 3 Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing, China, 4 Department of Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China, 5 Department of Neuropathology, Beijing Neurosurgical Institute, Beijing, China Abstract Objectives: Seizure is a common presenting symptom of primary brain tumors. There are no published studies regarding the roles of MicroRNA (miRNA) in tumor-related epilepsy. The authors set out to correlate miR-196b expression in low-grade glioma patients with pre-operative seizures and post-operative seizure control. Methods: Twenty-three patients with WHO grade II astrocytomas and 83 similar patients for independent validation were included. Follow-up visits regarding seizure prognosis were scheduled at 6 months. MiRNA profiling was used to identify differentially expressed miRNAs. The most important miRNA was determined by quantitative reverse-transcriptase polymerase chain reaction (q-PCR) in the validation cohort. Gene ontology (GO) analysis and whole genome mRNA profiling was performed to inve