spatiotemporal expression control correlates with intragenic scaffold matrix attachment regions (smars) in arabidopsis thaliana控制时空表达与基因内脚手架矩阵附件区域(smars)在拟南芥.pdfVIP

Spatiotemporal Expression Control Correlates with Intragenic Scaffold Matrix Attachment Regions (S/MARs) in Arabidopsis thaliana 1 1 2 4 1,3 1* Igor V. Tetko , Georg Haberer , Stephen Rudd , Blake Meyers , Hans-Werner Mewes , Klaus F. X. Mayer 1 GSF National Research Center for Environment and Health, MIPS, Institute for Bioinformatics, Neuherberg, Germany, 2 Bioinformatics Group, Turku Centre for ¨ ¨ Biotechnology, Tykistokatu, Turku, Finland, 3 Department of Genome-Oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universitat Munchen, Freising, Germany, 4 Department of Plant and Soil Sciences, Delaware Biotechnology Institute, Newark, New Jersey, United States of America Scaffold/matrix attachment regions (S/MARs) are essential for structural organization of the chromatin within the nucleus and serve as anchors of chromatin loop domains. A significant fraction of genes in Arabidopsis thaliana contains intragenic S/MAR elements and a significant correlation of S/MAR presence and overall expression strength has been demonstrated. In this study, we undertook a genome scale analysis of expression level and spatiotemporal expression differences in correlation with the presence or absence of genic S/MAR elements. We demonstrate that genes containing intragenic S/MARs are prone to pronounced spatiotemporal expression regulation. This characteristic is found to be even more pronounced for transcription factor genes. Our observations illustrate the importance of S/ MARs in transcriptional regulation and the role of chromatin structural characteristics for gene regulation.